Neural Organoids

Types	Forebrain, midbrain, or custom organoids.
Quality Control	Multiple QC assays
Delivery	Shipped at your desired timepoint.

Overview

Human 3D organoids are fast becoming the leading technology in disease modelling for research, drug development, and drug screening purposes. At NeuraCell, we have developed methods to produce human iPSC-derived organoids efficiently and at scale.

Our production begins with high quality iPSCs which we develop into organoids using directed differentiation with small molecules and growth factors. We use rigorous QC assays to ensure correct brain region-specific patterning. We have experience making over 12,000 organoids, and we can ship organoids anywhere in the US as live organoids, as lysates, or as RNA.

Using a collection of iPSC lines from the Tau Consortium collection (Karch et. al. 2019) with MAPT mutations (R406W, V337M, IVS10+16, and S305I). We have successfully generated forebrain organoids (Table 1) and demonstrated that they exhibit the expected developmental profile.

Single cell transcriptomic and studies demonstrate the expected pattern time course of development:

20 days – largely progenitor cells in rosettes expressing SOX2, PAX6, FOXG1 and EMX2

2 months – progenitor cells plus deep layer cortical neurons

4 months – fewer progenitor cells, deep layer and upper layer cortical neurons, astrocytes

6 months – few progenitor cells, more mature neurons and glia.

Mutation	Genotype	Cell Line
S305I	WT (Unedited Control line)	F13505.1-S2A3
S305I	S305I Het (Introduced Heterozygote)	F13505.1-I1B10
IVS10+16	WT/WT (Corrected Control)	GIH-36-C2d1E04
IVS10+16	WT/IVS10+16	GIH-36-C2
IVS10+16	WT/WT (Corrected Control)	GIH-178-C1d2A10
IVS10+16	WT/IVS10+16 (IVS10+16C>T)	GIH-178-C1
R406W	WT/WT (Corrected Control)	F11362.1d1C11
R406W	R406W/WT	F11362.1d1F10
V337M	WT/WT (Corrected Control)	GIH-6-C1d1E11
V337M	V337M/WT	GIH-6-C1d1A02
V337M	WT/WT (Corrected Control)	GIH-7-C2d2B12
V337M	V337M/WT	GIH-7-C2d2A01

Table 1. Tau Consortium iPSC lines already validated for organoid production
(see Karch et al, 2019; PMID: 31631020). Note: we can produce organoids from other iPSCs lines not on this list after optimizing (for an additional charge (see pricing below).

Organoid Production Process: A) Optimized methods to produce uniform sized organoids, B) One of our QC assays to check biomarkers via qPCR, C) a 20 µM section of a forebrain organoid immunoassayed for appropriate cortical layer markers for glutamatergic neurons – CTIP2, MEF2C and BRN2, scale bar: 20 µm. D) Low power image of a forebrain organoid in culture, scale bar: 100 µm. E) 30-day Midbrain organoid section (20 µm thick) immunostained for FOXA2 in green and Tyrosine Hydroxylase (TH) in red, scale bar: 100 µm. FOXA2-an early midbrain marker of midbrain progenitor cells; TH – a midbrain marker of dopaminergic neurons.

Organoids can be shipped at any desired timepoint (e.g. 2, 4, or 6 months). Pricing provided below if for non- profit academic institutions. Please contact us for pricing details for for-profit entities.

Organoid Production Costs (90 organoids) Academic (Non-profit) Pricing
Optimization study (2-month)*	$3,450
2-month Organoids	$3,450
4-month Organoids	$4,650
6-month Organoids	$5,825
Shipping and handling cost per order:	$200
only required for new iPSC lines not listed above*

Possible delivery options from one plate of 90 Organoids:

1 timepoint, 90 organoids
2 timepoints, 45 organoids per timepoint
3 timepoints, 30 organoids per timepoint

Neural Organoids

Overview

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