Description
Midbrain organoids are derived from 3D spheroids of human iPSCs. These cultures are exposed to key pattern factors (e.g. dual-SMAD inhibition, sonic hedgehog) to direct cells into midbrain cell fates.The timing of cell type maturation follows the expected time course of human cortical development. At 20 days, organoids are composed of midbrain progenitor cells (e.g. FOXA2+, LMX2+). By two months, organoids consist of progenitors and dopaminergic neurons (e.g. TH+) followed by maturation of astrocytes (e.g. AQP4+).
Neuracell has established quantifiable quality control (QC) checkpoints to identify well-patterned regionally specific organoids with emphasis on a fail-early approach to keep production costs down. Our QC’d well-patterned midbrain organoids reproducibly consistent across batches and organoid replicates.
